Sumários
Theoretical Class 3: Conformational Disorders; Tutorial Class
31 Outubro 2025, 17:00 • ANA PAULA COSTA DOS SANTOS PERALTA LEANDRO
Theoretical Class 3: Conformational Disorders
§ Protein aggregation
in the cell
§ Gain-of –function
conformational disorders
§ Amyloid fibrils
§ Disease mechanism
§ Examples
§ Loss-of-function
conformational disorders
§ Missense mutations
§ Disease Mechanism
§ Therapeutic
Approaches
§ Pharmacological
chaperones
§ Proteostasis
regulators
Tutorial Class
Laboratory 1 & 2
30 Outubro 2025, 17:00 • ANA PAULA COSTA DOS SANTOS PERALTA LEANDRO
L1 – SDS-PAGE analysis of target proteins
L2 – Compounds screening
Laboratory 1 & 2
29 Outubro 2025, 17:00 • ANA PAULA COSTA DOS SANTOS PERALTA LEANDRO
L1 – SDS-PAGE analysis of target proteins
L2 – Compounds screening
Theoretical Class 3: Protein Degradation in the Cell; Practical Class 1: Protein Characterization Introduction
28 Outubro 2025, 17:00 • ANA PAULA COSTA DOS SANTOS PERALTA LEANDRO
Theoretical Class 3: Protein Degradation in the Cell
§ Protein Turnover
§
PEST sequences
and N-degron
§ The
Ubiquitin-Proteasome System (UPS)
§
Ubiquitin; Protein Ubiquitination; The Proteasome
26S; The Ubiquitin Code
§ The Lysosomal
System
§
Autophagy Lysosome Pathways (ALPs): micro, macro
and chaperone mediated
§ Protein Degradation
in the Endoplasmic Reticulum (ERAD)
§ Protein Degradation
in the Mitochondria
§ Protein Quality
Control Systems (PQC)
§
Endoplasmic reticulum associated: Unfolded Protein
Response (UPR)
§
Cytoplasm Associated
§
Spatial Sequestration of Misfolded Proteins
Practical Class 1: Protein Characterization Introduction
§
Heterologous protein expression in eukaryotic and
prokaryotic cells:
§ Recombinant DNA
technology and expression systems
§
Protein characterization
§ Protein function
§ Biochemical and
Biophysical methods to study Protein Folding/Unfolding/Stability
Theoretical Class 1: Introduction to the Curricular Unit; Protein Homeostasis: From Structure to Function; Theoretical Class 2: Protein Folding in the Cell
27 Outubro 2025, 17:00 • ANA PAULA COSTA DOS SANTOS PERALTA LEANDRO
Theoretical
Class 1: Introduction to the Curricular Unit; Protein Homeostasis: From
Structure to Function
§
Introduction
§ Learning Objectives
§ Class Schedule
§ Evaluation
§
Protein Structure
§ Amino acids: Chemical and Structural Properties
§ The Peptide Bond
§ Levels of Structural Organization
§ Forces Stabilizing the Protein Structure
§ Post-Translational Modifications
§
Protein Folding &
Function
Theoretical
Class 2: Protein Folding in the Cell
§
Folding in vitro vs
Folding in vivo
§
Components of the
Proteostasis Network
§ Molecular Chaperones and Co-Chaperones
§ Molecular Chaperone Families
§
Protein Synthesis in the
Ribosome
§ Properties of the Exit Tunnel
§ Newly Synthesized Proteins and the Role of Molecular Chaperones
§
Co- and Post-translational
Folding in the Cytosol
§
Folding in cellular
compartments
§ Endoplasmic Reticulum (ER)
§ Mitochondria
§ Nucleus